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Image Search Results
Journal: Nature biomedical engineering
Article Title: Microscopy with ultraviolet surface excitation for rapid slide-free histology
doi: 10.1038/s41551-017-0165-y
Figure Lengend Snippet: a, Schematic diagram of MUSE optical design. An inverted microscope design is used, modified to include a UV-transparent stage and off-axis UV LED illumination. b,c, Unfixed ovine kidney, cut surface prepared via razor blade, stained with eosin and imaged with a grayscale camera, excited with c , 405-nm and d, 280-nm sources. Tubules and collecting ducts are easier to discern with 280-nm UV because of the latter’s surface-limited tissue penetration. d , 280-nm light excites multiple dyes that emit in the visible range due to the S 0 -S 2 excitation (shown in yellow) followed by S 1 -S 0 relaxation, excitation and emission spectra for DAPI and rhodamine are shown. e,f , Image captured from the cut surface of a thick, formalin-fixed porcine kidney specimen stained for 10 seconds with a mixed solution of rhodamine and Hoechst, and excited using a 280-nm UV LED. Multiple colours are visible: the muscular artery’s internal and external laminae (orange); collagen (blue); nuclei (lilac); tubules (orange and green). Endothelial cell nuclei extending down inside the artery are visible. Scale bars = 100 μm.
Article Snippet: The emitted fluorescent light was collected using a variety of long-working-distance objectives (e.g., 10X NA 0.28, Mitutoyo, Kawasaki, Japan, and 10X NA, 0.45 Nikon, Tokyo, Japan) and focused using an infinity-corrected tube lens (Thorlabs-ITL200, F = 200 mm, Thorlabs, Newton, New Jersey, USA) onto either a
Techniques: Inverted Microscopy, Modification, Staining